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healthy ipsc  (Axol Bioscience)


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    Axol Bioscience healthy ipsc
    Healthy Ipsc, supplied by Axol Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ipsc+derived+cms/Human+iPSC-Derived+Ventricular+Cardiomyocytes/pmc12519157-68-2-8
    Average 93 stars, based on 22 article reviews
    healthy ipsc - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Amelioration and treatment of infarction damage with a polypeptide segement of BNIP3
    Article Snippet: Cell lines The HL-1 cell line was provided by W. C. Claycomb (Louisiana State University Medical Center, New Orleans, LA, USA) and cultured as previously described (Claycomb et al., 1998). .. Human CMs were obtained from Axol Bioscience, UK, and cultured according to the manufacturer's instructions. ..

    Membrane:

    Article Title: Reverse engineering of BNIP3 identifies a mitochondrial protective peptide
    Article Snippet: H 2 O 2 generation was further determined in MCF-7 cells (15,000 cells per well, 2 μM STS, 52 μM B-017, 1 h) using the Invitrogen CellROX Deep Red Reagent (ThermoFisher Scientific, C10422 ), according to the manufacturer’s instructions, using the BMG FLUOstar Omega Microplate Reader. .. To analyse the mitochondrial inner membrane potential in human iPSC-derived cardiomyocytes (Axol 2505), the cells were incubated in HEPES buffer (113 mM NaCl, 4.7 mM KCl, 12 mM HEPES, 1.2 mM MgSO 4 , 30 mM taurine, and 1.3 mM CaCl 2 , pH 7.4) under 1% O 2 at 37 °C for 2 h. For reoxygenation, the cells were incubated in HEPES buffer supplemented with 5.5 mM glucose under 21% O 2 at 37 °C for 2 h. During hypoxia/reoxygenation the cardiomyocytes were treated with B-017 (4.4 μM) or the negative control peptide (4.4 μM). .. To determine the effect of B-017 on STS-induced changes in mitochondrial inner membrane potential, MCF-7 cells (15,000 cells per well) were incubated with 500 nM STS and 52 μM B-017 for 1 h. To analyse the effect of B-017 on doxorubicin-induced changes in mitochondrial inner membrane potential, human cardiomyocytes (7,500 cells per well HCM, PromoCell) were incubated with 10 μM DOX and 4.4 μM B-017 for 4 h. The iPSC-derived cardiomyocytes and MCF-7 cells were then incubated with 6 μM JC-1 dye in medium at 37 °C for 30 min and HCM with TMRM dye and processed according to the manufacturer’s instructions (JC-1 Kit, ThermoFisher Scientific, TMRM Kit, Abcam).

    Incubation:

    Article Title: Reverse engineering of BNIP3 identifies a mitochondrial protective peptide
    Article Snippet: H 2 O 2 generation was further determined in MCF-7 cells (15,000 cells per well, 2 μM STS, 52 μM B-017, 1 h) using the Invitrogen CellROX Deep Red Reagent (ThermoFisher Scientific, C10422 ), according to the manufacturer’s instructions, using the BMG FLUOstar Omega Microplate Reader. .. To analyse the mitochondrial inner membrane potential in human iPSC-derived cardiomyocytes (Axol 2505), the cells were incubated in HEPES buffer (113 mM NaCl, 4.7 mM KCl, 12 mM HEPES, 1.2 mM MgSO 4 , 30 mM taurine, and 1.3 mM CaCl 2 , pH 7.4) under 1% O 2 at 37 °C for 2 h. For reoxygenation, the cells were incubated in HEPES buffer supplemented with 5.5 mM glucose under 21% O 2 at 37 °C for 2 h. During hypoxia/reoxygenation the cardiomyocytes were treated with B-017 (4.4 μM) or the negative control peptide (4.4 μM). .. To determine the effect of B-017 on STS-induced changes in mitochondrial inner membrane potential, MCF-7 cells (15,000 cells per well) were incubated with 500 nM STS and 52 μM B-017 for 1 h. To analyse the effect of B-017 on doxorubicin-induced changes in mitochondrial inner membrane potential, human cardiomyocytes (7,500 cells per well HCM, PromoCell) were incubated with 10 μM DOX and 4.4 μM B-017 for 4 h. The iPSC-derived cardiomyocytes and MCF-7 cells were then incubated with 6 μM JC-1 dye in medium at 37 °C for 30 min and HCM with TMRM dye and processed according to the manufacturer’s instructions (JC-1 Kit, ThermoFisher Scientific, TMRM Kit, Abcam).

    Negative Control:

    Article Title: Reverse engineering of BNIP3 identifies a mitochondrial protective peptide
    Article Snippet: H 2 O 2 generation was further determined in MCF-7 cells (15,000 cells per well, 2 μM STS, 52 μM B-017, 1 h) using the Invitrogen CellROX Deep Red Reagent (ThermoFisher Scientific, C10422 ), according to the manufacturer’s instructions, using the BMG FLUOstar Omega Microplate Reader. .. To analyse the mitochondrial inner membrane potential in human iPSC-derived cardiomyocytes (Axol 2505), the cells were incubated in HEPES buffer (113 mM NaCl, 4.7 mM KCl, 12 mM HEPES, 1.2 mM MgSO 4 , 30 mM taurine, and 1.3 mM CaCl 2 , pH 7.4) under 1% O 2 at 37 °C for 2 h. For reoxygenation, the cells were incubated in HEPES buffer supplemented with 5.5 mM glucose under 21% O 2 at 37 °C for 2 h. During hypoxia/reoxygenation the cardiomyocytes were treated with B-017 (4.4 μM) or the negative control peptide (4.4 μM). .. To determine the effect of B-017 on STS-induced changes in mitochondrial inner membrane potential, MCF-7 cells (15,000 cells per well) were incubated with 500 nM STS and 52 μM B-017 for 1 h. To analyse the effect of B-017 on doxorubicin-induced changes in mitochondrial inner membrane potential, human cardiomyocytes (7,500 cells per well HCM, PromoCell) were incubated with 10 μM DOX and 4.4 μM B-017 for 4 h. The iPSC-derived cardiomyocytes and MCF-7 cells were then incubated with 6 μM JC-1 dye in medium at 37 °C for 30 min and HCM with TMRM dye and processed according to the manufacturer’s instructions (JC-1 Kit, ThermoFisher Scientific, TMRM Kit, Abcam).



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    doi: 10.1101/2025.09.24.678132

    Figure Lengend Snippet: Generation and characterization of iPSC-derived cardiomyocytes. A. Schematic overview of the experimental design. B. Reprogramming factor expression in control and MRPL44 patient-derived iPSCs after reprogramming (OCT4; green, NANOG; red and TRA-1-60; green) (Scale bar; 200µm) C. Cardiac marker positivity of iPSC derived CMs. Immunofluorescence, cardiac troponin (cTnT; green) and nuclei (DAPI, blue) (Scale bar; 50 µm). D. Purification of iPSC derived cardiomyocytes. Representative FACS analysis shows 91.9% cTnT positive cells after purification.

    Article Snippet: Contracting cells were observed from day 8 onwards. iPSC derived CMs were purified using magnetic beads as per manufacturer’s instructions (#130-110-188, Miltenyi Biotec) on day 15-20 of differentiation.

    Techniques: Derivative Assay, Expressing, Control, Marker, Immunofluorescence, Purification

    Impaired lipid homeostasis in patient CMs A. Lipid droplet analysis. Immunostaining of neutral lipid droplets in MRPL44 and Control iPSC-CMs by Lipidtox. (cTnT, yellow; Lipidtox, red; TOM20, green; DAPI, blue; scale: 50 µm) B. Quantification of droplets per cell between patient and control CMs. (n=1273 CMs) *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001 (Student’s t-test) C. Gene expression levels of lipid transporter CD36, LDLR, ACSL1 and PDK4 gene in different media conditions. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001 (Wald test)

    Journal: bioRxiv

    Article Title: Nutrient-dependent pathology in mitochondrial hypertrophic cardiomyopathy model

    doi: 10.1101/2025.09.24.678132

    Figure Lengend Snippet: Impaired lipid homeostasis in patient CMs A. Lipid droplet analysis. Immunostaining of neutral lipid droplets in MRPL44 and Control iPSC-CMs by Lipidtox. (cTnT, yellow; Lipidtox, red; TOM20, green; DAPI, blue; scale: 50 µm) B. Quantification of droplets per cell between patient and control CMs. (n=1273 CMs) *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001 (Student’s t-test) C. Gene expression levels of lipid transporter CD36, LDLR, ACSL1 and PDK4 gene in different media conditions. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001 (Wald test)

    Article Snippet: Contracting cells were observed from day 8 onwards. iPSC derived CMs were purified using magnetic beads as per manufacturer’s instructions (#130-110-188, Miltenyi Biotec) on day 15-20 of differentiation.

    Techniques: Immunostaining, Control, Gene Expression

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    Journal: International Journal of Bioprinting

    Article Title: Blood-derived biomaterials for tissue graft biofabrication by solvent-based extrusion bioprinting

    doi:

    Figure Lengend Snippet: 3D bioprinting of specific tissue constructs using bioinks functionalized with blood-derived biomaterials

    Article Snippet: Kumar et al. (2019); ACS Biomater Sci Eng [ ] , 1 mL of the bioink: 990 μL of fibrinogen solution (60 mg/mL), 155 mg of gelatin- furfuryl at 25°C Heat it at 37 °C for 1 h with stirring, add Rose Bengal (10 μL) Polymerization: 50 units/mL thrombin, and 80 mM CaCl 2 , Human-induced pluripotent stem cell (iPSC)-derived cardiomyocytes (CM) Human CM AC 16 cell lines (ATCC) , Fibrinogen Thrombin (#) , SEM, swelling assay, rheology, Fourier transform infrared spectroscopy (FTIR), biocompatibility Cardiac cell-laden constructs To validate the applicability of this scaffold toward mimicking cardiac tissue.

    Techniques: Construct, Formulation, Migration, In Vivo, Clinical Proteomics, In Vitro, Micro-CT, Concentration Assay, Gene Expression, Transplantation Assay, Activation Assay, Immunohistochemistry, Immunohistochemical staining, Fourier Transform Infrared Spectroscopy, Spectroscopy, Functional Assay, In Situ, Modification, Activity Assay, Expressing, Marker, Comparison